Sequence comparison of satellite-less centromeres with orthologous non-centromeric regions in equids

Author: bela sepiashvili
Keywords: centromeres, evolutionary new centromeres, onagro
Annotation:

Nowadays the research of centromeres function is an actual issue since the structure and function of centromeres is not fully defined yet. The presence of satellite DNA in the given regions of chromosomes makes it difficult for the complete genome analysis of centromeres. The recent studies have detected satellite-free centromeres , so-called evolutionary new centromeres, (ENCs), which do not contain satellite DNA. Evolutionary new centromeres provide a unique opportunity in centromeres research. The aim of the abovementioned Master’s thesis was to study: the CENP-A protein enriched DNA sequences in onager ( Equus hemionus onager) chromosomes; Also, the detailed analysis of centromere sequences on the examples of two centromeres of onager (EHO22 and EHO 27). The cellular lines of onager fibroblasts were used as the study material. Based on the conducted research, the centromere and so-called secondary peaks were determined in the onager genome. These peaks were analyzed according to the horse reference genome. Also the sequence analysis of DNA segments of the centromere regions of the 22nd and 27th chromosomes has been carried out.

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სატელიტებისაგან თავისუფალი ცენტრომერების ნუკლეოტიდური თანმიმდევრობების შედარება ორთოლოგიურ არაცენტრომერულ უბნებთან ცხენისებრთა ოჯახის წარმომადგენლებში [ka]