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The study of influence DPPC and DPPA liposomes with Gentamicin on the E.coli bacteria by physical methods

Author: juna sulashvili
Keywords: Gentamycin, DPPC and DPPA liposomes with Gentamicin, turbidimeter
Annotation:

An observation on the bacterial growth process and an exploration of the impact of the different kind of medicines on the bacteria is very important. Our research is about the reproduction of E.coli and its attitude with different external factors. We used the antibiotic Gentamycin and the complex liposomes like DPPA and DPPC, which have been made according to our new method. In our researches, for determining the efficiency of the antimicrobial agents, we used the turbidimeter, which gave us the opportunity to observe the changing the number of bacteria on line by the measuring of the density of solution. This method also allows us to explore the reasons of the pausing of the growth of bacteria in the stationary phase. We have demonstrated that there is a lot of nutrient area in the solution and the pausing of the reproduction of bacteria is caused by other factors. Our research shows that using of influence DPPC and DPPA liposomes with Gentamicin is more effective than the using of the pure Gentamycin itself. The use of antibiotics in the complex with liposomes has the advantage that the concentration of antibiotics is low in liposomes and, at the same time, the delivery of antibiotic in the place with minimal losses.



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