Nanoparticles and PLGA nanoparticles and their thermodynamic properties

Author: Ana Kakabadze
Keywords: Nanoparticles, Nanotechnology, NanoParticles for drug delivery
Annotation:

Biodegradable nanoparticles formulated from poly (D,L-lactide-co-glycolide) (PLGA) have been extensively investigated for sustained and targeted / localized delivery of different agents including plasmid DNA, proteins and peptides and low molecular weight compounds. Research about the mechanism of intracellular uptake of nanoparticles, their trafficking and sorting into different intracellular compartments, and the mechanism of enhanced therapeutic efficacy of nanoparticle-encapsulated agent at cellular level is more recent and is the primary focus of the review. Recent studies in laboratory demonstrated rapid escape of PLGA nanoparticles from the endo-lysosomal compartment into cytosol following their uptake. Based on the above mechanism, various potential applications of nanoparticles for delivery of therapeutic agents to the cells and tissue are discussed. The influence of conditions of preparation on the thermal stability of hermetic poly (lactic-coglycolic) acid (PLGA) nanoparticles was investigated using calorimetry. The hermetic destruction temperature depends on the PLA/PGA ratio. The maximum temperature of decapsulation of the nanoparticles occurs at a PLA/PGA ratio of 85/15. The temperature of hermetic destruction does not depend on the PVA and PLGA solutions’ mixing temperature during preparation, nor on the homogenization temperature.

Lecture files:

ნანონაწილაკები და PLGA ნანონაწილაკების თერმოდინამიკური თვისებები [ka]