Defining different composition PLGA nanoparticles stability in time and temperature using calorimetry method

Author: Gvantsa Tonia
Co-authors: Tamaz Mdzinarashvili; Mariam Khvedelidze; Eka Shekiladze;Aljoscha Koenneke; Marc Schneider
Keywords: Nanoparticles, stabilizers, stability, time, temperature, nanocomplexes
Annotation:

In the following thesis we have used different types of stabilizers to prepare PLGA nanoparticles. Stabilizers influence on nanoparticle stability and drug delivery kinetics. PLGA (Poly lactic-co-glycolic acid) itself is made by complex of PLA (Poly lactic acid ) and PGA (Poly glycolic acid) synthetic molecules and stabilizers. In general, these kind of nanoparticles diameter is 100 – 200 nm in size. We have prepared PLGA with the following stabilizers: PL 35, Pl 10RS and DMAP and determined nanoparticles structural changes caused by external factors; we have also measured stability of nanoparticles in time and with variations in temperature. We used calorimetric measurements to determine nanoparticle stability. Until then our attention was devoted to the role of stabilizers in nanoparticle stability. Another purpose of the research was to determine structural changes of PLGA nanoparticles and hydrophobic nature Tetrandrine complex, which were caused by Tetrandrine drug insertion in PLGA nanoparticles. We have determined PLGA-Tetrandrine complex structural changes using the Differential scanning calorimeter and spectrophotometers :SPECTRO UV-UIS DUAL BEAM 8 AUTO CELL (UVS-2800) and Lambda 38 (PerkinElmer, Rodgau, Germany)). Nanoparticles were centrifugated on Heraeus Multifuge X1R Centrifuge (Thermo Fisher Scientific, Waltham, MA, USA). Analysed data provides important results. It appears that nanoparticles’ (pure and with drugs) structural changes depend not only on the stabilizers, but on temperature and time as well. Thesis based on joint research of Faculty of Exact and Natural Sciences, Department of Physics, Ivane Javakhishvili Tbilisi State University and Biopharmaceutics and Pharmaceutical Technology, Department of Pharmacy, Saarland University, Saarbrücken, Germany.