Evaluation antitumor effectiveness of adamantine derivates in MEC1 cell lines

Author: Nino Maisuradze
Keywords: Adamantine derivatives, anticancer chemotherapy, Chronic Lymphocytic Leukaemia, immunophenotyping.
Annotation:

In XXI century cancer is still one of the major problems in medicine, the death rate index caused by cancer remains high. Among them is noteworthy Chronic Lymphocytic Leukemia – for which the main method of treatment is chemotherapy, on this basis it’s more and more important to discover and create highly effective and less toxic new generation of anticancer medications. One of the subject of special interest are therapeutic organic compounds which include adamantane derivatives and have wide spectrum of pharmacological activity. The goal of the research is to study biological activities of the compounds based on adamantane, examination their effectiveness and safety in MEC1 cancer in vitro model. Adamantane derivatives which were included in the study were provided by Tbilisi State University Organic Chemistry laboratory, which were selected due to their anticancer activities, by the use of mathematical modeling. Remarkably even existence of adamantane radical in the drag in most cases increase its activity and decrease toxicity. Within the study MEC1 leukemic cells were cultivated with the research compound, characteristics of cell cycle and apoptosis were studied by flow cytometry with different time and dosage regime, in parallel were prepared cytopreps. As a result of research, confirmed anticancer activities of adamantane based compounds, safety acceptable dose limit was evaluated during the examination of the low concentrations of derivatives and confirmed prolonged positive therapeutic effect of the compounds.